Category: Protocols

Standard PCR/Gel-isolation/Digestion/Phenol-Extraction

  1- PCR mixture (50 l) 10X Pfu buffer (Stratagene#600153-82):                  5.0 10 mM dNTPs (Roche #11922505001):               1.5        (single use aliquots) Plasmid template (50 ng/l):                                  1.0 Primer1 (from IDT 25 mM in TE’):                         […]

DNA oligonucleotide 5’-labelling with 32P

  1- Prepare 5X Kinasing Buffer: 1 M Tris-HCl pH 7.5:                  12.5   uL 1 M MgCl2:                               2.5      uL 1M DTT:                                   1.25    uL 10 mM Spermidine:                    2.5      uL 200 mM EDTA:                         2.5       […]

Widal Test

The Test Widal Test is an agglutination test which detects the presence of serum agglutinins (H and O) in patients serum with typhoid and paratyphoid fever. When facilities for culturing are not […]

Vector Preparation

  Digest NcoI/XhoI/CIP/Extract pET21b-nH6-TEV-cPKA/pET21d/… 1- Digest a total of ~ 3 ug of vector in a final volume of 50 uL. 10U NcoI/XhoI mix 10X NEB2 (NEB):          5.0 2 mg/ml BSA (NEB):      […]