Standard PCR/Gel-isolation/Digestion/Phenol-Extraction

  1- PCR mixture (50 l) 10X Pfu buffer (Stratagene#600153-82):                  5.0 10 mM dNTPs (Roche #11922505001):               1.5        (single use aliquots) Plasmid template (50 ng/l):                                  1.0 Primer1 (from IDT 25 mM in TE’):                         2.5 Primer2 (from

Read more

DNA oligonucleotide 5’-labelling with 32P

  1- Prepare 5X Kinasing Buffer: 1 M Tris-HCl pH 7.5:                  12.5   uL 1 M MgCl2:                               2.5      uL 1M DTT:                                   1.25    uL 10 mM Spermidine:                    2.5      uL 200 mM EDTA:                         2.5       uL H2O:                                         30.75   

Read more