Standard PCR/Gel-isolation/Digestion/Phenol-Extraction

  1- PCR mixture (50 l) 10X Pfu buffer (Stratagene#600153-82):                  5.0 10 mM dNTPs (Roche #11922505001):               1.5        (single use aliquots) Plasmid template (50 ng/l):                                  1.0 Primer1 (from IDT 25 mM in TE’):                         2.5 Primer2 Read More …