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Bioactive metabolites from the leaves of Withania adpressa.

A new interesting article has been published in Pharm Biol. 2018 Dec;56(1):505-510. doi: 10.1080/13880209.2018.1499781. and titled:

Bioactive metabolites from the leaves of Withania adpressa.

Authors of this article are:

Ben Bakrim W, El Bouzidi L, Nuzillard JM, Cretton S, Saraux N, Monteillier A, Christen P, Cuendet M, Bekkouche K.

A summary of the article is shown below:

CONTEXT: Withania (Solanaceae) species are known to be a rich source of withanolides, which have shown several biological properties.OBJECTIVE: To identify the compounds responsible for Withania adpressa Coss. antioxidant activity and further test them for their NF-κB inhibition and antiproliferative activity in multiple myeloma cells.MATERIALS AND METHODS: Compounds were obtained from the EtOAc extract of W. adpressa leaves. Structure elucidation was carried out mainly by 1D- and 2D-NMR, and mass spectrometry. Isolated compounds were tested in a dose-response for their in vitro NF-κB inhibition and antiproliferative activity in multiple myeloma cells after 5 and 72 h treatment, respectively.RESULTS: The fractionation resulted in the isolation of a new glycowithanolide named wadpressine (5) together with withanolide F, withaferin A, coagulin L, and nicotiflorin. The latter showed a moderate ability to scavenge free radicals in DPPH (IC50 = 35.3 µM) and NO (IC50 = 41.3 µM) assays. Withanolide F and withaferin A exhibited low µM antiproliferative activity against both multiple myeloma cancer stem cells and RPMI 8226 cells. Furthermore, they inhibited NF-κB activity with IC50 values of 1.2 and 0.047 µM, respectively. The other compounds showed a moderate inhibition of cell proliferation in RPMI 8226 cells, but were inactive against cancer stem cells and did not inhibit NF-κB activity.DISCUSSION AND CONCLUSIONS: One new glycowithanolide and four known compounds were isolated. Biological evaluation data gave further insight on the antitumor potential of withanolides for refractory cancers.

Check out the article’s website on Pubmed for more information:

This article is a good source of information and a good way to become familiar with topics such as:

NF-κB inhibition;Withanolides;antioxidant activity;antiproliferative activity;wadpressine


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