Nanobody immunostaining for correlated light and electron microscopy with preservation of ultrastructure.
Authors of this article are:
Fang T, Lu X, Berger D, Gmeiner C, Cho J, Schalek R, Ploegh H, Lichtman J.
A summary of the article is shown below:
Morphological and molecular characteristics determine the function of biological tissues. Attempts to combine immunofluorescence and electron microscopy invariably compromise the quality of the ultrastructure of tissue sections. We developed NATIVE, a correlated light and electron microscopy approach that preserves ultrastructure while showing the locations of multiple molecular moieties, even deep within tissues. This technique allowed the large-scale 3D reconstruction of a volume of mouse hippocampal CA3 tissue at nanometer resolution.
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