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Nanobody immunostaining for correlated light and electron microscopy with preservation of ultrastructure.

A new interesting article has been published in Nat Methods. 2018 Nov 5. doi: 10.1038/s41592-018-0177-x. [Epub ahead of print] and titled:

Nanobody immunostaining for correlated light and electron microscopy with preservation of ultrastructure.

Authors of this article are:

Fang T, Lu X, Berger D, Gmeiner C, Cho J, Schalek R, Ploegh H, Lichtman J.

A summary of the article is shown below:

Morphological and molecular characteristics determine the function of biological tissues. Attempts to combine immunofluorescence and electron microscopy invariably compromise the quality of the ultrastructure of tissue sections. We developed NATIVE, a correlated light and electron microscopy approach that preserves ultrastructure while showing the locations of multiple molecular moieties, even deep within tissues. This technique allowed the large-scale 3D reconstruction of a volume of mouse hippocampal CA3 tissue at nanometer resolution.

Check out the article’s website on Pubmed for more information:



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