Anti-adhesion of probiotic Enterococcus faecium WEFA23 against 5 pathogens and the beneficial effect of its S-layer proteins against Listeria monoc…
Authors of this article are:
He Y, Xu X, Zhang F, Xu D, Liu Z, Tao X, Wei H.
A summary of the article is shown below:
Enterococcus faecium WEFA23 is a potential probiotic strain isolated from Chinese infant feces. In this study, the antagonistic activity of E. faecium WEFA23 on the adhesion to pathogens was investigated. E. faecium WEFA23 was able to compete, exclude and displace the adhesion of Escherichia coli O157:H7, Salmonella Typhimurium ATCC13311, Listeria monocytogenes CMCC54007, Staphylococcus aureus CMCC26003 and Shigella sonnei ATCC25931 on Caco-2 cells. Among them, L. monocytogenes achieved the strongest inhibition rate in both competition and displacement assays. Those anti-adhesion capacities were related to the bacterial physicochemical properties (hydrophobicity, auto-aggregation and co-aggregation) of bacterial surface. For L. monocytogenes, the anti-adhesion capacity was affected by the heat-treatment, cell density and growth phase of E. faecium WEFA23, 108 cfu/mL of viable cells at the stationary phase exhibited the strongest anti-adhesion activity. In addition, removal of S-layer proteins of E. faecium WEFA23 by treatment with 5 M LiCl significantly decreased its adhesion capacity, and those S-layer proteins were able to compete, displace and exclude L. monocytogenes at different levels. Both cells and S-layer proteins of E. faecium WEFA23 significantly reduced the apoptosis of Caco-2 cells induced by L. monocytogenes, which was mediated by caspase-3 activation. This study might be helpful to understand the anti-adhesion mechanism of probiotics against pathogens.
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