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Simultaneous determination of alkaloids dicentrine and sinomenine in Stephania epigeae by 1H NMR spectroscopy.

A new interesting article has been published in J Pharm Biomed Anal. 2018 Oct 25;160:330-335. doi: 10.1016/j.jpba.2018.08.007. Epub 2018 Aug 6. and titled:

Simultaneous determination of alkaloids dicentrine and sinomenine in Stephania epigeae by 1H NMR spectroscopy.

Authors of this article are:

Dong JW, Li XJ, Cai L, Shi JY, Li YF, Yang C, Li ZJ.

A summary of the article is shown below:

Stephania epigaea Lo is an important herbal medicine used as antiphlogistic and analgesic drugs. Its major components are dicentrine (1) and sinomenine (2). In the present study, a rapid, accurate, and precise method for simultaneous quantitation of dicentrine (1) and sinomenine (2) in S. epigeae using 1H NMR spectra was developed. The deuterated solvent of DMSO-d6 enabled satisfactory separation of the signals to be integrated in 1H NMR spectrum and dimethyl terephthalate was selected as an internal standard. The feature signals of δ 7.57 and 5.70 were selected for quantifying the dicentrine (1) and sinomenine (2), respectively. Validation of the quantitative method was performed in terms of specificity, accuracy, precision, and stability. This work implied that quantitative 1H NMR represents a feasible alternative to high-performance liquid chromatography-based methods for quantitation of dicentrine (1) and sinomenine (2) in S. epigeae and is suitable for the quality control of S. epigeae.

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This article is a good source of information and a good way to become familiar with topics such as:

Determination;Dicentrine;Quantitative (1)H NMR;Sinomenine;Stephania epigeae

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