A Cell Surface-Anchored Ratiometric DNA Tweezer for Real Time Monitoring of Extracellular and Apoplastic pH.
Authors of this article are:
Zeng S, Liu D, Li C, Yu F, Fan L, Lei C, Huang Y, Nie Z, Yao S.
A summary of the article is shown below:
Precise and dynamic imaging of extracellular pH is one of crucial yet challenging tasks for studying cell physiological and pathological processes. Here we construct a DNA tweezer to dynamically monitor pH change of cellular microenvironment. The DNA tweezer contains three key elements: a 3 strands ssDNA-frame labelled with cholesterol to anchor on cell membrane, a pH sensitive i-motif sequence in the middle to dynamically control the switch of “open” and “close” state of the DNA tweezer, and a pair of FRET fluorophores (rhodamine green and rhodamine red) separately on the two arms of the tweezer to reflect its state. With cholesterol, a natural component of cell membrane, as an anchoring element, the sensor exhibited high cell membrane insertion efficiency and low cytotoxicity. Using i-motif as a sensing element, it can fast and reversibly respond to extracellular pH in the pH range of 5.0-7.5, and further perform real time imaging of cell-surface pH change with excellent spatial and temporal resolution. Moreover, apoplastic pH change during the alkalization process of plant roots caused by RALF1 (rapid alkalinization factor) was directly detected by the sensor, demonstrating the potential applications of the sensor in cell biology, biomedical research and plant tissue engineering.
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