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Lectin AtGAL1 interacts with high-mannose glycoform of the purple acid phosphatase AtPAP26 secreted by phosphate-starved Arabidopsis.

A new interesting article has been published in Plant Cell Environ. 2018 Oct 20. doi: 10.1111/pce.13463. [Epub ahead of print] and titled:

Lectin AtGAL1 interacts with high-mannose glycoform of the purple acid phosphatase AtPAP26 secreted by phosphate-starved Arabidopsis.

Authors of this article are:

Ghahremani M, Park J, Anderson EM, Marty-Howard NJ, Mullen RT, Plaxton WC.

A summary of the article is shown below:

Among 29 predicted Arabidopsis purple acid phosphatases (PAPs), AtPAP26 functions as the principle extra- and intracellular PAP isozyme that is upregulated to recycle and scavenge Pi during Pi-deprivation or leaf senescence. Our companion paper documented the co-purification of a secreted, high-mannose AtPAP26-S2 glycoform with AtGAL1 (At1g78850), a Pi starvation-inducible (PSI) and Galanthus nivalis agglutinin-related (mannose-binding) and apple domain lectin. This study tests the hypothesis that AtGAL1 binds AtPAP26-S2 to modify its enzymatic properties. Far-western immunodot blotting established that AtGAL1 readily associates with AtPAP26-S2, but not the low mannose AtPAP26-S1 glycoform, nor other secreted PSI PAPs (i.e., AtPAP12 or AtPAP25). Analytical gel filtration indicated that 55 kDa AtGAL1 and AtPAP26-S2 polypeptides associate to form a 112 kDa heterodimer. Microscopic imaging of transiently-expressed, fluorescent protein-tagged AtGAL1, and associated bimolecular fluorescence complementation assays demonstrated that: (i) like AtPAP26, AtGAL1 also localizes to lytic vacuoles of Pi-deprived Arabidopsis, and (ii) both proteins interact in vivo. AtGAL1 preincubation significantly enhanced the acid phosphatase activity and thermal stability of AtPAP26-S2, but not AtPAP26-S1. We hypothesize that AtGAL1 plays an important role during Pi deprivation through its interaction with mannose-rich glycans of AtPAP26-S2, and consequent positive impact on AtPAP26-S2 activity and stability.

Check out the article’s website on Pubmed for more information:



This article is a good source of information and a good way to become familiar with topics such as:

Arabidopsis thaliana (thale cress);glycobiology;glycoforms;lectins;phosphorus limitation;protein-protein interactions;purple acid phosphatase

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