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Defining CRISPR-Cas9 genome-wide nuclease activities with CIRCLE-seq.

A new interesting article has been published in Nat Protoc. 2018 Oct 19. doi: 10.1038/s41596-018-0055-0. [Epub ahead of print] and titled:

Defining CRISPR-Cas9 genome-wide nuclease activities with CIRCLE-seq.

Authors of this article are:

Lazzarotto CR, Nguyen NT, Tang X, Malagon-Lopez J,, Guo JA, Aryee MJ,, Joung JK, Tsai SQ.

A summary of the article is shown below:

Circularization for in vitro reporting of cleavage effects by sequencing (CIRCLE-seq) is a sensitive and unbiased method for defining the genome-wide activity (on-target and off-target) of CRISPR-Cas9 nucleases by selective sequencing of nuclease-cleaved genomic DNA (gDNA). Here, we describe a detailed experimental and analytical protocol for CIRCLE-seq. The principle of our method is to generate a library of circularized gDNA with minimized numbers of free ends. Highly purified gDNA circles are treated with CRISPR-Cas9 ribonucleoprotein complexes, and nuclease-linearized DNA fragments are then ligated to adapters for high-throughput sequencing. The primary advantages of CIRCLE-seq as compared with other in vitro methods for defining genome-wide genome editing activity are (i) high enrichment for sequencing nuclease-cleaved gDNA/low background, enabling sensitive detection with low sequencing depth requirements; and (ii) the fact that paired-end reads can contain complete information on individual nuclease cleavage sites, enabling use of CIRCLE-seq in species without high-quality reference genomes. The entire protocol can be completed in 2 weeks, including time for gRNA cloning, sequence verification, in vitro transcription, library preparation, and sequencing.

Check out the article’s website on Pubmed for more information:



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