NIR two-photon fluorescent probe for biothiol detection and imaging of living cells in vivo.
Authors of this article are:
Xia X , Qian Y .
A summary of the article is shown below:
A fluorescence probe, Cz-BDP-NBD, for detecting biothiols with two photon excited fluorescence has been designed and used under irradiation from sapphire pulsed lasers at 800 nm. Upon addition of biothiols, NBD was removed through a SNAr reaction and the fluorophore Carbazyl BODIPY (Cz-BDP) was released simultaneously. A two photon excited fluorescence was turned on and accompanied by a strong red emission at 660 nm, the two-photon absorption (TPA) cross section of the Cz-BDP reached a value of 198.5 GM at 800 nm. Moreover, Hcy/Cys and GSH could be distinguished between by a two emission channel, which resulted from the different mechanisms. For Hcy and Cys, N-NBD-Hcy and N-NBD-Cys were formed through a 5/6-membered cyclic transition, they displayed distinct fluorescent turn-on signal changes at 540 nm (Φ = 0.28, Φ = 0.24) and 670 nm, while GSH only has a single emission channel at 670 nm. Therefore, the Cz-BDP-NBD can distinguish GSH, Hcy and Cys with a fast response time (within one minute), high selectivity and sensitivity. In addition, it has been successfully applied for the detection of Cys/Hcy and GSH in living cells and ratio imaging. The Cz-BDP-NBD probe could be used for discriminative sensing of intracellular Cys, Hcy and GSH, and has the potential for use in biological applications.
Check out the article’s website on Pubmed for more information:
This article is a good source of information and a good way to become familiar with topics such as:
Categories: Science News