Super-resolution Geometric Barcoding for Multiplexed miRNA Profiling.
Authors of this article are:
Xu W,, Yin P, Dai M.
A summary of the article is shown below:
MicroRNA (miRNA) expression profiles hold promise as biomarkers for diagnostics and prognosis of complex diseases. Herein, we report a super-resolution fluorescence imaging-based digital profiling method for specific, sensitive, and multiplexed detection of miRNAs. In particular, we applied the DNA-PAINT (point accumulation for imaging in nanoscale topography) method to implement a super-resolution geometric barcoding scheme for multiplexed single-molecule miRNA capture and digital counting. Using synthetic DNA nanostructures as a programmable miRNA capture nano-array, we demonstrated high-specificity (single nucleotide mismatch discrimination), multiplexed (8-plex, 2 panels), and sensitive measurements on synthetic miRNA samples, as well as applied one 8-plex panel to measure endogenous miRNAs levels in total RNA extract from HeLa cells.
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This article is a good source of information and a good way to become familiar with topics such as:
DNA nanotechnology;DNA-PAINT;microRNA;multiplexing;super-resolution imaging
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