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Benzophenone used as the photochemical reagent for pinpointing C=C locations in unsaturated lipids through shotgun and liquid chromatography-mass s…

A new interesting article has been published in Anal Chim Acta. 2018 Oct 22;1028:32-44. doi: 10.1016/j.aca.2018.04.046. Epub 2018 Apr 21. and titled:

Benzophenone used as the photochemical reagent for pinpointing C=C locations in unsaturated lipids through shotgun and liquid chromatography-mass s…

Authors of this article are:

Xu T, Pi Z, Song F, Liu S, Liu Z.

A summary of the article is shown below:

Unsaturated lipids exhibit different physiological significances due to the different locations of the carbon-carbon double bond (C=C). Identifying lipid isomers with mass-based methods remains challenging. Xia’s group has been successfully employed Paternò-Büchi (PB) reaction, a photochemical reaction with UV irradiation, coupled with tandem mass spectrometry (MS/MS) to identify and quantify unsaturated lipids in complex mixtures. However, the existing PB reagents possess certain demerits. In this regard, a new PB reagent that is compatible with various lipidomic analysis platforms must be screened. In this study, we comprehensively evaluated the conditions of the PB reaction and screened benzophenone as a new PB reagent. Benzophenone possesses unique advantages, such as a relative high PB yield; the PB products could be readily distinguished from the reacted lipids based on the added high molecular weight (182 Da); and the benzophenone does not affect the lipids appearance interval for reversed-phase column separation. Furthermore, we optimized the reaction conditions by using benzophenone as a PB reagent and summarized the molecular formulas of the diagnostic ions according to the fragment rules. The proposed PB method has been implemented in shotgun and LC-MS lipidomics. To our best knowledge, this work is the first to report the integrated PB reaction with LC-MS lipidomics for identification of lipid isomers.

Check out the article’s website on Pubmed for more information:



This article is a good source of information and a good way to become familiar with topics such as:

Benzophenones;Carbon;Chromatography, Liquid;Isomerism;Kinetics;Lipids;Mass Spectrometry;Models, Molecular;Molecular Conformation;Oxygen

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