Proteomics analysis to compare the venom composition between Naja naja and Naja kaouthia from the same geographical location of eastern India: Corr…
Authors of this article are:
Chanda A, Patra A, Kalita B, Mukherjee AK.
A summary of the article is shown below:
BACKGROUND: Cobra bite is frequently reported across the Indian subcontinent and is associated with a high rate of death and morbidity. In eastern India (EI) Naja naja and N. kaouthia are reported to be the two most abundant species of cobra.RESEARCH DESIGN AND METHODS: The venom proteome composition of N. naja (NnV) and N. kaouthia (NkV) from Burdwan districts of EI were compared by separation of venom proteins by 1D-SDS-PAGE followed by LC-MS/MS analysis of protein bands. The potency of commercial polyantivenom (PAV) was assessed by neutralization, ELISA, immuno-blot and venom-PAV immunoaffinity chromatography studies.RESULTS: Proteomic analysis identified 52 and 55 proteins for NnV and NkV, respectively, when searched against the Elapidae database. A small quantitative difference in venom composition between these two species of cobra was observed. PAVs exhibited poor cross-reactivity against low molecular mass toxins (<20 kDa) of both cobra venoms, which was substantiated by a meagre neutralization of their phospholipase A2 activity. 3FTx and phospholipase A2, the two major classes of non-enzymatic and enzymatic proteins, respectively, were partially recognized by PAVs.CONCLUSIONS: Efforts must be made to improve immunization protocols and supplement existing antivenoms with antibodies raised against the major toxins of these venoms.
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This article is a good source of information and a good way to become familiar with topics such as:
Antivenomics;cobra venom;snake venom;snakebite;venom-antivenom cross-reactivity
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