Development of a homogeneous time-resolved fluorescence assay for detection of viral double-stranded RNA.
Authors of this article are:
Fujita M, Adachi K, Nagasawa M.
A summary of the article is shown below:
The group of positive-sense single-stranded RNA ((+) ssRNA) viruses includes many important human pathogens. However, specific antiviral agents are not currently available for many RNA viruses. For screening of antiviral agents, methods that are simple, rapid, and compatible with high-throughput are required. Here, we describe a novel method for measurement of double-stranded RNA using a homogeneous time-resolved fluorescence assay. This method allowed detection of human rhinovirus (HRV), enterovirus, coxsackievirus, and murine norovirus. Furthermore, this method detected antiviral activity of a HRV 3C protease inhibitor. The assay may be useful for discovery of antiviral agents against (+) ssRNA viruses.
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This article is a good source of information and a good way to become familiar with topics such as:
Antiviral agents;Double-stranded RNA;Homogeneous time-resolved fluorescence assay;RNA virus;Viral replication
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