Bile salt hydrolase and S-layer protein are the key factors affecting the hypocholesterolemic activity of Lactobacillus casei-fermented milk in ham…
Authors of this article are:
Guo CF, Zhang S, Yuan YH, Li JY, Yue TL.
A summary of the article is shown below:
SCOPE: Lactobacillus casei F0822-fermented milk has exhibited significant hypocholesterolemic activity in hamsters in our previous study. Under this premise, the objective of this study was to further explore whether bile salt hydrolase (BSH) and S-layer protein (SLP) of the strain have a significant influence on hypocholesterolemic activity of the fermented milk.METHODS AND RESULTS: Independent and double interposon mutants of BSH and SLP genes were constructed from wild-type L. casei F0822 via chromosomal insertion of chloramphenicol or/and erythromycin resistance genes based on double-crossover homologous recombination. The mutants- and the wild-type strain-fermented milk was prepared (viable counts of approximately 8.0 × 108 colony-forming units/mL each) and intragastrically administered to high-cholesterol-fed hamsters once daily at a dose of 1.25 mL/d for 28 d, respectively. Both the BSH-deficient mutant- and the SLP-deficient mutant-fermented milk significantly (p < 0.05) increased serum total and LDL-cholesterol levels in hamsters compared with the wild-type strain-fermented milk. However only the BSH-deficient mutant-fermented milk could significantly (p < 0.05) increase hepatic total and esterified cholesterol levels in hamsters.CONCLUSION: Both BSH and SLP had a significant influence on the hypocholesterolemic activity of L. casei F0822-fermented milk in hamsters. Nevertheless, the BSH was greater than the SLP in this regard. This article is protected by copyright. All rights reserved.This article is protected by copyright. All rights reserved.
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This article is a good source of information and a good way to become familiar with topics such as:
Lactobacillus casei;S-layer protein;bile salt hydrolase;cholesterol;hypocholesterolemic activity
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