Science News

Control of Lineage-Specific Gene Expression by Functionalized gRNA Barcodes.

A new interesting article has been published in ACS Synth Biol. 2018 Oct 19;7(10):2468-2474. doi: 10.1021/acssynbio.8b00105. Epub 2018 Sep 17. and titled:

Control of Lineage-Specific Gene Expression by Functionalized gRNA Barcodes.

Authors of this article are:

Al’Khafaji AM, Deatherage D, Brock A.

A summary of the article is shown below:

Lineage tracking delivers essential quantitative insight into dynamic, probabilistic cellular processes, such as somatic tumor evolution and differentiation. Methods for high diversity lineage quantitation rely on sequencing a population of DNA barcodes. However, manipulation of specific individual lineages is not possible with this approach. To address this challenge, we developed a functionalized lineage tracing tool, Control of Lineages by Barcode Enabled Recombinant Transcription (COLBERT), that enables high diversity lineage tracing and lineage-specific manipulation of gene expression. This modular platform utilizes expressed barcode gRNAs to both track cell lineages and direct lineage-specific gene expression.

Check out the article’s website on Pubmed for more information:



This article is a good source of information and a good way to become familiar with topics such as:

barcode;clonal dynamics;heterogeneity;lineage tracing;population variation

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