A chimeric aptamers-based and MoS2 nanosheet-enhanced label-free fluorescence polarization strategy for ATP detection.
Authors of this article are:
Fan YY, Mou ZL, Wang M, Li J, Zhang J, Dang F, Zhang ZQ.
A summary of the article is shown below:
Adenosine triphosphate (ATP) as a primary energy source plays an unique role in the regulation of all cellular events. The necessity to detect ATP requires sensitive and accurate quantitative analytical strategies. Herein, we present our study of developing a MoS2 nanosheet-enhanced aptasensor for fluorescence polarization-based ATP detection. A bifunctional DNA strand was designed to consist of chimeric aptamers that recognize and capture ATP and berberine, a fluorescence enhancer. In the absence of ATP, the DNA strand bound to berberine will be hydrolyzed when Exonuclease I (Exo I) is introduced, releasing berberine as a result. On the contrary, when ATP is present, ATP aptamer folds into a G-quadruplex structure, thus the complex can resist degradation by Exo I to maintain berberine for fluorescent detection purpose. In addition, to magnify the fluorescence polarization (FP) signal, MoS2 nanosheets were also adopted in the system. This nanosheets-enhanced FP strategy is simple and facile which does not require traditional dye-labeled DNA strands and complex operation steps. The developed fluorescence polarization aptasensor showed high sensitivity for the quantification of ATP with a detection limit of 34.4 nM, performing well both in buffer solution and in biological samples.
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