Science News

[Vancomycin-based fluorescent enzyme-linked immunoabsorbent assay for detection of Staphylococcus aureus].

A new interesting article has been published in Sheng Wu Gong Cheng Xue Bao. 2018 Sep 25;34(9):1510-1517. doi: 10.13345/j.cjb.170488. English Abstract and titled:

[Vancomycin-based fluorescent enzyme-linked immunoabsorbent assay for detection of Staphylococcus aureus].

Authors of this article are:
Li S Chen C Chen X.

A summary of the article is shown below:
in English, Chinese以猪IgG 作为捕获抗体固定金黄色葡萄球菌,修饰有万古霉素的量子点荧光微球作为“检测抗体”,建立荧光酶联免疫吸附法检测金黄色葡萄球菌。文中制备了平均粒径为100 nm 的量子点荧光微球并与万古霉素偶联;摸索了反应最佳盐离子浓度为0.01 mol/L,反应最佳pH 为6.0。在该实验条件下,金黄色葡萄球菌的检测灵敏度为10⁴ CFU/mL,与其他致病菌无交叉反应。以上结果表明,该方法可用于快速检测金黄色葡萄球菌,为金黄色葡萄球菌的临床监控和食品检测提供参考。.In the study, fluorescent enzyme-linked immnoabsorbent assay for detection of Staphylococcus aureus was established with IgG from pig as capture antibody and quantum dot nanobeads (QBs) labeled vancomycin (QB-Vans) as testing antibody. Quantum dot of about 100 nm partical size nanobeads were prepared and linked with vancomycin. The optimum concentrations of salt ions were 0.01 mol/L, and the optimum pH was 6.0. Under the optimum conditions, the detection sensitivity for S. aureus was 10⁴ CFU/mL, and there was no cross-reaction with other pathogenic bacteria. Thus, the method could be used for rapid screening of S. aureus, for the clinical monitoring and foodborne pathogens detection.

Check out the article’s website on Pubmed for more information:

This article is a good source of information and a good way to become familiar with topics such as: Staphylococcus aureus;fluorescent enmyze-linked immunosorbent assay;pig IgG;quantum dot nanobeads;vancomycin.

Categories: Science News