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Red-shifted fluorogenic substrate for detecting lacZ-positive cells in living tissue with single-cell resolution.

A new interesting article has been published in Angew Chem Int Ed Engl. 2018 Sep 25. doi: 10.1002/anie.201808670. and titled:

Red-shifted fluorogenic substrate for detecting lacZ-positive cells in living tissue with single-cell resolution.

Authors of this article are:
Ito H Kawamata Y Kamiya M Tsuda-Sakurai K Tanaka S Ueno T Komatsu T Hanaoka K Okabe S Miura M Urano Y.

A summary of the article is shown below:
The Escherichia coli lacZ gene encoding β-galactosidase is a widely used reporter, but few synthetic substrates are available for detecting its activity with single-cell resolution in living samples. Our recently reported fluorogenic substrate SPiDER-βGal is suitable for this purpose, but its hydrolysis product shows green fluorescence emission, and a red-shifted analogue is required for use in combination with green fluorescent protein (GFP) markers. Here, we describe the development of a red-shifted fluorogenic substrate for β-galactosidase, SPiDER-Red-βGal, based on silicon rhodol scaffold and by using a carboxylic group as the intramolecular nucleophile. LacZ-positive cells were successfully labeled with SPiDER-Red-βGal at the single cell resolution in living samples, and enabled us to visualize different cell types in combination with GFP markers.

Check out the article’s website on Pubmed for more information:



This article is a good source of information and a good way to become familiar with topics such as: Fluorescent probes;lacZ;quinone methide;single-cell resolution;β-galactosidase.

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