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Insights into cholesterol/membrane protein interactions using paramagnetic solid-state NMR.

A new interesting article has been published in Chemistry. 2018 Sep 25. doi: 10.1002/chem.201804550. and titled:

Insights into cholesterol/membrane protein interactions using paramagnetic solid-state NMR.

Authors of this article are:
Jaipuria G Giller K Leonov A Becker S Zweckstetter M.

A summary of the article is shown below:
Cholesterol is an essential component of animal cell membranes and impacts the structure and function of membrane proteins. But how cholesterol exerts its functions remains often enigmatic. Here we show that high-resolution solid-state NMR in combination with paramagnetic cholesterol analogues is a powerful approach to study the interaction of membrane proteins with cholesterol. Application of the method to the 169-residue protein TSPO provides residue-specific information about its interaction with cholesterol. Comparison with NMR signal perturbations induced by diamagnetic cholesterol furthermore supports changes in the structure of mammalian TSPO caused by cholesterol binding.

Check out the article’s website on Pubmed for more information:



This article is a good source of information and a good way to become familiar with topics such as: NMR spectroscopy;lipid;membrane protein;protein structure.

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