A high-throughput system used to determine frequency and distribution of phosphine resistance across large geographical regions.
Authors of this article are:
Schlipalius DI Tuck AG Pavic H Daglish GJ Nayak MK Ebert PR.
A summary of the article is shown below:
BACKGROUND: Next-generation sequencing can enable genetic surveys of large numbers of individuals. We developed a genotyping-by-sequencing assay for detecting strong phosphine resistance alleles in the dihydrolipoamide dehydrogenase (dld) gene of Rhyzopertha dominica populations. The assay can estimate the distribution and frequency of resistance variants in thousands of individual insects in a single run.RESULTS: We analysed 1,435 individual insects collected over a 1 year period from 59 grain storage sites including farms (n=29) and central storages (n=30) across eastern Australia. Resistance alleles were detected in 49% of samples, 38% of farms and 60% of central storages. Although multiple alleles were detected, only two resistance variants (P49S and K142E) were widespread and each appeared to have a distinct but overlapping geographical distribution.CONCLUSION: The structure type in which the grain is stored had a strong effect on resistance allele frequency. We observed higher frequencies of resistance alleles in bunker storages at central sites compared to other storage types. This contributed to the higher frequencies of resistance alleles in bulk handling facilities relative to farms. The discovery of a storage structure that pre-disposes insects to resistance highlights the utility of our high throughput assay system for improvement of phosphine resistance management practices. This article is protected by copyright. All rights reserved.This article is protected by copyright. All rights reserved.
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This article is a good source of information and a good way to become familiar with topics such as: Phosphine;fumigation;insecticide;resistance.
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