Discovery of multi-megabase polymorphic inversions by chromosome conformation capture sequencing in large-genome plant species.
Authors of this article are:
Himmelbach A Ruban A Walde I Šimková H Doležel J Hastie A Stein N Mascher M.
A summary of the article is shown below:
Chromosomal inversions occur in natural populations of many species and may underlie reproductive isolation and local adaptation. Traditional methods of inversion discovery are labor-intensive and lack sensitivity. Here, we report the use of three-dimensional contact probabilities between genomic loci as assayed by chromosome conformation capture sequencing (Hi-C) to detect multi-megabase polymorphic inversions in four barley genotypes. Inversions are validated by fluorescence in situ hybridization and Bionano optical mapping. We propose Hi-C as a generally applicable method for inversion discovery in natural populations. This article is protected by copyright. All rights reserved.
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This article is a good source of information and a good way to become familiar with topics such as: Hordeum vulgare ;Barley;chromosomal inversions;chromosome conformation capture sequencing;genomics;optical mapping.
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