Arabidopsis phytochrome A nuclear translocation is mediated by a far red elongated hypocotyl 1 – importin complex.
Authors of this article are:
Helizon H Rösler-Dalton J Gasch P von Horsten S Essen LO Zeidler M.
A summary of the article is shown below:
Phytochrome A (phyA) is a red and far-red sensing photoreceptor regulating plant growth and development. Its biologically active far-red (FR) absorbing form Pfr translocates into the nucleus and subsequently regulates gene expression. Two transport facilitators, far red elongated hypocotyl 1 (FHY1) and FHY1-like (FHL), are crucial for its cytoplasmic-nuclear translocation. FHY1 interacts preferentially with activated phyA (Pfr) in assays with recombinant phyA and FHY1 and in vivo. Nuclear translocation of the phyA-FHY1 complex depends on a nuclear localization signal of FHY1, which is recognized by IMPαs independently of phyA. The complex is guided along the actin cytoskeleton. Additionally, FHY1 has the ability to exit the nucleus via the exportin route thus is able to repeatedly transport phyA molecules to the nucleus, balancing the nucleo-cytoplasmic distribution. The direction of FHY1s transport appears to depend on its phosphorylation state in different compartments. Phosphoprylated serins close to the nuclear localization signal prevent FHY1 binding to IMPα. The work presented here elucidates key steps of the mechanism by which photoactivated phyA translocates to the nucleus. This article is protected by copyright. All rights reserved.
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