Methylamine Salts Agar Recipe

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  1. Dissolve the following in 900 ml distilled water
    • 1g KH2PO4
    • 2.12g K2HPO4
    • 6.75g Methylamine HCl
    • 15g agar
    • 5ml Solution A
    • 1ml Solution B
  2. Adjust pH to 7.0
  3. q.s. to 1L with distilled water
  4. Autoclave to sterilize

Solution A

  1. Dissolve to following in 95 ml distilled water
    • 2g MgSO4-7H2O
    • 0.2g CaCl2-2H2O
    • 0.2g FeSO4-7H2O
  2. q.s. to 100 ml with distilled water

Solution B

  1. Dissolve the following in 95 ml distilled water
    • 0.05g MnSO4-7H2O
    • 0.05g Na2MoO4-2H2O
  2. q.s. to 100 ml with distilled water