Mannitol Agar Recipe

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  1. Dissolve 3g peptone in 850ml of distilled water
  2. Dissolve 5g yeast extract in the solution from step 1
  3. Dissolve 25g of mannitol in the solution from step 2
  4. Dissolve 15g of agar in the solution from step 3
  5. Bring to 1000ml with distilled water
  6. Autoclave or filter sterilize