Soluble Expression of Transcription Factor Brf1 in E. coli

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This protocol describes the expression of S. cerevisiae’s transcription factor Brf1 as a soluble protein in E. coli. The protocol uses protein stabilizers, low temperature and low IPTG induction. The protocol can be adapted to the expression of fragments of Brf1 as well as Brf1 originating from different species. The protocol uses a BL21(DE3)-pLysS as host strain.