Purification of His-Tagged Transcription Factor Brf1

Purification of His-Tagged Transcription Factor Brf1
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The protocol describes an effective procedure for the purification of histidine-tagged transcription factor Brf1, expressed in E.coli as a soluble fraction (using protocol PROT-0001A). The protocol provides masses and volumes to be used as guidelines for any scale up or scale down of the procedure.

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